Picky - Your Magic PRM and SRM Designer Tool

1. Database Query

Real Time Correction Settings

2. Retention Time Adjustment

3. Additional Settings



Stable isotope label

Dwell Time

15.02.2020 Prosit support
Prosit msp files can now alternatively be uploaded as a library. Please note: The filtering of overhead peptides is based on a random process, since scores or quality measures are not provided with Prosit predictions.
16.09.2020 FAIMS support
FAIMS column based on an in house CV predictor is available. Please use with care. The CVs/predictions can be different and less accurate in other labs.
1.07.2020 TargetDIA
A new acquisition mode, which combines peptides into floating DIA windows.
28.04.2020 Rerelease.
Newest version moved to Server.
28.04.2020 Fragment Mass Conversion
Fragment masses are converted according to settings in the SILAC selection panel and written to the SpectraTable and TransitionTable text files. The msms.txt export files are not affected.
01.02.2020 Exploris Support
Thermo Exploris specific export has been added. In addition, protein specific injection times are provided based on the observed average abundance in the proteome tools database.
01.04.2020 Second Step Correction
RT prediction has been extended in order to provide more precise RT adjustment. In case the uploaded calibration file contains overlapping sequences with the Picky Database a two step adjustment is executed. The gradient will be re-adjusted considering the observed average RT-shifts among sequence pairs across the gradient. Further, RT specific windows are estimated considering the set quantile limits. With 't+' a RT-spacer is defined, in order to prevent too small windows. This option can be disabled with the 'Drift correction' trigger
25.09.2019 Updated Database:
Recompiled Picky Database from the official ProteomeTools spectral library release. Picky now stores information for different collision energies for HCD spectra and includes more peptides.
13.11.2018 Fixed a bug which kept cterminal peptides with a missed cleavage site in the selection even if miss-cleavages should be excluded.
10.05.2018 Extended Isotopic Labeling:
Implemented medium R and K. Single R and K isotopes can be selected.
10.04.2018 Thermo Fusion Lumos
Thermo Fusion Lumos Template is now available in the export selection.

This application was developed by the Selbach lab. Please contact us (henrik.zauber(at)mdc-berlin.de) if you have feature requests or problems. If you are using Picky, please cite us: Zauber et al. (2018). Nature Methods. http://doi.org/10.1038/nmeth.4607 . The Picky spectrum library is based on ProteomeTools (Zolg et al. (2017) Nature Methods). At its current state, 24540 proteins covered by 352608 sequences and 1041410 spectra are implemented in the Picky database. This application uses the shiny, RSQLite, data.table and shinyBS R-packages. Copyright 2017
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Matthias Selbach
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