Fragment masses are converted according to settings in the SILAC selection panel and written to the SpectraTable and TransitionTable text files. The msms.txt export files are not affected.
01.02.2020 Exploris Support
Thermo Exploris specific export has been added. In addition, protein specific injection times are provided based on the observed average abundance in the proteome tools database.
01.04.2020 Second Step Correction
RT prediction has been extended in order to provide more precise RT adjustment. In case the uploaded calibration file contains overlapping sequences with the Picky Database a two step adjustment is executed. The gradient will be re-adjusted considering the observed average RT-shifts among sequence pairs across the gradient. Further, RT specific windows are estimated considering the set quantile limits. With 't+' a RT-spacer is defined, in order to prevent too small windows. This option can be disabled with the 'Drift correction' trigger
25.09.2019 Updated Database:
Recompiled Picky Database from the official ProteomeTools spectral library release. Picky now stores information for different collision energies for HCD spectra and includes more peptides.
13.11.2018 Fixed a bug which kept cterminal peptides with a missed cleavage site in the selection even if miss-cleavages should be excluded.
10.05.2018 Extended Isotopic Labeling:
Implemented medium R and K. Single R and K isotopes can be selected.
10.04.2018 Thermo Fusion Lumos
Thermo Fusion Lumos Template is now available in the export selection.
This application was developed by the
lab. Please contact us (henrik.zauber(at)mdc-berlin.de) if you have feature requests or problems.
If you are using Picky, please cite us:
Zauber et al. (2018). Nature Methods. http://doi.org/10.1038/nmeth.4607
The Picky spectrum library is based on ProteomeTools (Zolg et al. (2017) Nature Methods). At its current state,
proteins covered by
spectra are implemented in the Picky database.
This application uses the
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